Table 2. Summary of binding properties for some Cyclin A2 alleles in human HEK 293 cells.
CDK 1(%) | CDK2(%) | p21(%) | p107(%) | |
WT | 100 | 100 | 100 | 100 |
D171A | 100 | 96 | 94,4 | 95 |
M200A | 100 | 100 | 89,5 | 57,4 |
L204A | 0 | 12,6 | 4,1 | 5,3 |
W207A | 0 | 5 | 0,5 | 0 |
Triple (M200A,L204A, W207A) | 0 | 0 | 0 | 0 |
Data are represented as percentage relative to WT control taken as 100% upon normalization to Cyclin A2 immunoprecipitation efficiency using densitometric analysis.