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. 2011 Jul 28;6(7):e22907. doi: 10.1371/journal.pone.0022907

Figure 5. Eos decreases the interaction between the LCR and the γ-globin region promoters.

Figure 5

(A–B) The human β-globin locus was analyzed by 3C assay in K562 cells that were (A) untransfected or transfected with pcDNA3.1-Eos, or (B) that were untransfected or transfected with pcDNA3.1-Eos and hemin-induced for 48 h. The relative positions of β-like globin genes are represented as black arrows along the top of each graph. The thick line (1) indicates the position and size of the fixed fragment 1 (LCR HS2/3/4), and the thin lines (2–11) show the position and size of other XbaІ fragments. Data are presented as mean ± SEM (n = 3) cross-linking frequencies between the LCR (HS2/3/4) and differentiation fragments, Error bars represent one standard deviation. *P<0.05.