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. 2011 Jul 28;6(7):e22907. doi: 10.1371/journal.pone.0022907

Figure 6. Enforced expression of Eos inhibited the rise in γ-globin gene expression during erythroid differentiation of CD34+ HPCs.

Figure 6

(A) The Eos mRNA level was analyzed by quantitative real-time PCR during Epo-induced erythroid differentiation of UCB-derived CD34+ HPCs. (B–C) The Eos mRNA level was analyzed by (B) RT-PCR and (C) quantitative real-time PCR in the erythroid induction culture of CD34+ HPCs that were untreated, infected with lentiviruses carrying the pWPXL vector, or infected with recombinant pWPXL-Eos lentiviruses. (D) Histograms illustrating globin expression as determined by quantitative real-time PCR in CD34+ HPCs induced into erythroid differentiation for 3, 7, 11, or 15 d. Quantitative real-time PCR experiments were performed in triplicate and were normalized to β-actin mRNA levels. The relative expression of each mRNA is depicted as the fold value in mRNA level compared to untreated CD34+ HPCs. Error bars represent one standard deviation. *P<0.05, #P<0.01.