Figure 8. Expression of genes involved in cuticle formation in wounded and unwounded plants.

Leaves were wounded and maintained for 1.5 h under high humidity in tightly covered well-watered trays (humid) or in uncovered trays at room conditions (dry) prior to expression of BDG and LACS2.3 genes. Gene expression was determined 0, 15 or 30 min after wounding in plants incubated under humid or dry conditions and either mock-inoculated (M) or inoculated with B. cinerea (Bc) (n = 3; ±SD). The experiment was carried out twice with similar results. The expression levels of unwounded control plants behaved similarly under dry or humid conditions. Different letters above each bar represent statistically significant differences (Dunn's test; P<0.05).