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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Br J Haematol. 2011 May 20;154(4):502–511. doi: 10.1111/j.1365-2141.2011.08738.x

Fig. 4.

Fig. 4

Haem transfer from MetHb to HSA inhibits protein oxidation. 100 μM MetHb was incubated in the presence or absence of 600 μM HSA for 4 h at 37 °C. These treatments were diluted 10-fold with serum-free medium and added to BAECs that had been pretreated with 10 μM Bt-AA for 1 h. After 4 h, BAECs were collected and the labelling of proteins modified by Bt-AA peroxidation products were visualized (n=3). Lane 1: Control lysate in the absence of Bt-AA. Lane 2: Control lysate in the presence of 10 μM Bt-AA. Lane 3: Cell lysate treated with 60 μM HSA in the presence of 10 μM Bt-AA. Lane 4: Cell lysate treated with10 μM metHb in the presence of 10 μM Bt-AA. Lane 5: Cell lysate treated with10 μM metHb + 60 μM HSA in the presence of 10 μM Bt-AA.