Figure 5.

BRC5 overexpression inhibits the completion of cell division. (a) BRC4 (4.23) and BRC5 (5.10) clones were treated with doxycycline, and bi- or multinucleated cells were counted from phase-contrast images taken at the indicated time points. The mean±s.e.m. from three independent experiments is shown. The number of cells enumerated in each experiment was ⩾500 cells. Similar results were obtained with two additional, independently derived myc-BRC5-expressing clones (Supplementary Figure S4). (b) Myc-BRC4- or -BRC5-expressing clones were followed by time-lapse imaging. The time taken from anaphase onset to the completion of cell division was measured and plotted in a box-and-whisker graph. The median value is shown; the bottom and top of the box represent the 25 and 75th percentiles, respectively. Statistical comparisons in this and the subsequent panels were by one-way analysis of variance using the Kruskal–Wallis test. See also Supplementary Movies 3 and 4. (c) Clone 5.10 is compared with HeLa TetOn cells before they were treated with doxycycline. (d) HeLa TetOn cells are compared before/after doxycycline treatment.