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Cyclic nucleotide stimulated VASP phosphorylation in intact human platelets. Cells were incubated with different concentrations of cyclic nucleotide analogs for the time points indicated. Proteins were separated by SDS-PAGE and subjected to western blot analysis for VASP phosphorylation with PKG-specific pVASP antibody 16C2 (a) or PKA-specific pVASP antibody 5C6 (b)
