Table 1.

Binding specificities of non-acetylated cGMP and cAMP analogs measured with the cGMP- and cAMP-EIA, respectively, from ENZO

Analog	Absolute binding EC50 [M] non-acetylated	Relative binding EC50cGMP/EC50Analog	Relative binding EC50cAMP/EC50Analog
cGMP	1.17 × 10−08	1.00
2′-dcGMP (17)	5.28 × 10−09	2.21
8-Br-cGMP (15)	1.83 × 10−09	6.40
8-Br-PET-cGMP (22)	1.36 × 10−07	0.08
cAMP	4.2 × 10−09		1.00
2′-dcAMP (6)	1.9 × 10−08		0.22

The EC₅₀ values represent means (SEM) of three experiments in duplicate