Fig. 8.

Imaging of MPP⁺-induced ROS production in VM dopaminergic neurons. Imaging of DCFDA/TH staining were performed at 48 h after treatment with MPP⁺. ROS production was detected by DCFDA staining. DCFDA: green fluorescence; TH: red fluorescence. Co-localization of DCFDA and TH was observed by merging red and green fluorescence signals. DCFDA 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate; Scale bar, 20 μm