Table 1. Primers used for the RT–PCR and PCR analysis of chBMC and sperm genomic DNA.

Gene	Gene ID	Forward/reverse	Sequences 5′–3′	Product size
GAPDH	NM204305	Forward	CCT TCA TCG ATC TGA ACT ACA TGG	265
		Reverse	GGA GCT GAG ATG ATA ACA CGC TTA
eGFP	EU530626	Forward	GAC TTC AAG GAG GAC GGC AAC A	256
		Reverse	TCT CGT TGG GGT CTT TGC TCA G
NeoR	EU530626	Forward	ACG TTG TCA CTG AAG CGG GAA G	442
		Reverse	GCA ATA TCA CGG GTA GCC AAC G
Nanog	NM001146142	Forward	AGA GAG TCA GTG GGT GGA TAA AGG	406
		Reverse	GGA GAG CTC GAG AAC TGT GTG ATA
SOX2	AB092842	Forward	GTG AAC CAG AGG ATG GAC AGT TAC	399
		Reverse	TTC TGG TAC TTC AGC ACC TGG TAG
C-kit	D13225	Forward	TCA GTG GGA GTT AAT GAT TCT GGA	242
		Reverse	TGC TCT TCC TTT TCT GAA GTG ATG
DAZL	AY211387	Forward	ATA CAA CTA TCA GGC TCC ACC ACA	304
		Reverse	TGC TCT TCC TTT TCT GAA GTG ATG
STRA8	XM416179	Forward	TGA AAA ACA AAC AAT GGA AGA AGA	441
		Reverse	CTA GAC AAT CCC TGA GTC TCG TTT
PAX6	NM205066	Forward	ACC AAC GAT AAC ATA CCC AGT GTG	429
		Reverse	TCC TAT TTC TCT GCA GCT TCC TTT
GATA4	NM204860	Forward	GAT TAT TCA GAA GGG CGA GAA TGT	394
		Reverse	TAA GAC TCT CAC TGC TGG ATG GAC
Brachyury	U67086	Forward	ATC ACA AAG ACA TGA TGG AGG AAG	423
		Reverse	GGA CCA TAA GTT CGG GTA CTG ACT
ALDBP	XM413860	Forward	GAG GTC TAT GAG CAG GGA GTG AA	410
		Reverse	TCC TCT TCT GGC AAC AGG TAC TC