Table 2. Semen analysis of eGFP BMCs-transferred recipients.
| Parameters | Normal | KU-16 | KU-17 | KU-18 | KU-19 |
|---|---|---|---|---|---|
| Sperm concentration (108/ml) | 207±32a | 7.1±0.9c | 4.2±1.7c | 91±15b | 102±11b |
| Semen volume (μl) | 350±90a | 162±28c | 143±22c | 211±39b | 190±40b |
| Viability (%) | 85±6a | 39±6b | 44±6b | 35±5c | 52±8b |
| Total sperm number (108/ejaculate) | 72.45±2.88a | 1.15±0.025c | 0.6±0.04c | 19.2±0.58b | 19.4±0.44b |
| Fertilization rate (%) | 85±4a | 32±11c | 37±8b | 41±9b | 54±5b |
| % of eGFP-positivea sperm | 0 | 0.69 | 0.82 | 0.08 | 0.01 |
a
Detection of eGFP-positive spermatozoa was done by immunocytochemistry analysis of ejaculated frozen semen.
a,b,cStatistical analyses were carried out by Student's t-test, and means with different superscripts within the same columns were significantly different (P<0.05).