Table 3. Germline chimera production by transfer of GFP BMC into the testes of normal recipients.
Age of |
Semen produced | eGFP gene detection in sperm | No. of hatched progenies | No. (%) of eGFP expressed progenies | Remarks | |
---|---|---|---|---|---|---|
Donor eGFP rooster (weeks) | Recipient rooster ID (weeks) | |||||
8 | KU-10 (8) | X | — | — | — | Dead after surgery |
8 | KU-12 (16) | X | — | — | — | Testis regression |
16 | KU-15 (16) | O | O | — | — | Dead before testcross |
16 | KU-16 (16) | O | O | 92 | 1 (1.1) | eGFP chicks produced |
30 | KU-17 (16) | O | O | 104 | 2 (1.9) | eGFP chicks produced |
30 | KU-18 (16) | O | O | 86 | 0 | eGFP gene integrated sperm |
30 | KU-19 (16) | O | O | 97 | 0 | eGFP gene integrated sperm |
30 | KU-20 (40) | X | — | — | — | No sperm production |
30 | KU-21 (40) | O | X | — | — | No eGFP sperm production |
O, positive; X, negative.