Table 1.
ATP inhibition of wild-type and mutant channels
| IC50, Mg-free ATP (μm) | n | IC50, MgATP (μm) | % unblocked current, 3 mm MgATP | n | |
|---|---|---|---|---|---|
| WT | 10.1 ± 0.6 | 9 | 15.7 ± 0.9 | 0.6 ± 0.1 | 26 |
| hetW68R | 27.6 ± 2.3 | 9 | 40.4 ± 6.5 | 3.1 ± 0.6 | 12 |
| homW68R | 73.7 ± 8.0 | 7 | 142 ± 12 | 7.9 ± 0.8 | 13 |
| homW68E | 153 ± 18 | 5.8 ± 1.1 | 7 | ||
| homW68K | 256 ± 29 | 10.0 ± 1.2 | 10 | ||
| homW68A | 228 ± 39 | 8.7 ± 1.5 | 6 | ||
| homW68C | 837 ± 338 | 29 ± 4 | 6 | ||
| homW68L | 107 ± 17 | 5.8 ± 0.5 | 7 | ||
| homW68M | 77 ± 13 | 5.9 ± 1.0 | 5 | ||
| homW68F | 1027 ± 490 | 30 ± 6 | 11 | ||
| homW68Y | >10 mm | 4 | >10 mm | 87 ± 1 | 8 |
| Kir6.2ΔC | 175 ± 25 | 5.4 ± 0.6 | 5 | ||
| Kir6.2ΔC-W68Y | 1.8 ± 0.4 mm | 34 ± 6 | 5 |
Data are the mean of the fits of the Hill equation (eqn 1) to the individual dose–response curves. IC50, half-maximal inhibitory concentration of ATP. n, number of patches.