Figure 5. Pharmacological characterization of the sustained depolarization—non-specific blockers of I_CAN/I_TRPC suppressed the sustained depolarization.

A, internal Ca²⁺ stores were first depleted with CPA (50 μm). VGCC-mediated rises in [Ca²⁺]_i were elicited with current-injected spikes and paired with puffs of DHPG. Addition of both flufenamate (FFA; 100 μm) and SKF96365 (30 μm) suppressed both the depolarization and the hyperpolarization (n = 5; P < 0.01, t test). B, addition of FFA (100 μm) alone blocked the depolarization (n = 3). C, addition of SKF96365 (30–100 μm) alone had no effect on either the depolarization or the hyperpolarization under these conditions (n = 5).