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. 2011 Apr 11;589(Pt 13):3231–3246. doi: 10.1113/jphysiol.2011.206748

Figure 1. Expression of K+ channel subunit mRNAs in pulmonary artery of WT and TASK-1/3 KO (KO) mice.

Figure 1

A, product from 35 PCR cycles using TASK-1 (left) or TASK-3 (right) primers, visualised with ethidium bromide. B, product from 32 PCR cycles using primers targeting K2P (top row), KCNQ (middle row) and delayed rectifier (bottom row) channel subunits in addition to β-actin, visualised with ethidium bromide. The presence (+) and absence (–) of reverse transcriptase is indicated above each lane. L50: DNA ladder 50bp; L100: DNA ladder 100bp. The expected product size is indicated to the right of each gel electrophoresis image. C, average densitometry values of the PCR products (n = 3). No statistical difference was detected between strains (t test).