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. 2011 May 3;589(Pt 13):3349–3369. doi: 10.1113/jphysiol.2011.209619

Table 2.

Primers used in real time RT-PCR analysis

Gene Accession number Primers (F; forward primer, R; reverse primer)
Beclin-1 NM_053739 F: 5′CGCCTCCTATTCCATCAAAA3′
R: 5′AACTGTGAGGACACCCAAGC3′
Atg5 AM087012 F: 5′AGGCTCAGTGGAGGCAACAG3′
R: 5′CCCTATCTCCCATGGAATCTTCT3′
Atg7 NM_001012097 F: 5′GCAGCCAGCAAGCGAAAG3′
R: 5′TCTCATGACAACAAAGGTGTCAAA3′
Atg12 NM_001038495 F: 5′GGCCTCGGAGCAGTTGTTTA3′
R: 5′CAGCATCAAAACTTCTCTGA3′
p53 NM_030989 F: 5′GCGTTGCTCTGATGGTGA3′
R: 5′CAGCGTGATGATGGTAAGGA3′
β2M NM_012512 F: 5′CGTGATCTTTCTGGTGCTTGTC3′
R: 5′TTCTGAATGGCAAGCACGAC3′