Figure 1.

Experimental protocols for ex vivo murine infarction studies in C57BL6 (WT) and RIP2^−/−. All hearts were subjected to 30 min of stabilization, 30 min of global ischaemia, followed by 120 min of reperfusion at the end of which the infarct size was determined by triphenyl tetrazolium chloride staining. For the first protocol, muramyl dipeptide (MDP) was perfused 15 min before ischaemia (I-R MDP precond). In a second protocol, MDP was perfused immediately at the onset of reperfusion in the presence or absence of the TAK1 inhibitor (5Z-7-oxozeanol, 1 µmol/L).