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Binding affinities for J1/2 mutants.
| RNA | Kd (nM) | Fold decrease | ΔΔGbind (kcal/mol) |
|---|---|---|---|
| Wild-typea | 190 ± 8.6 | 1 | 0 |
|
| |||
| C15A/C17A | 580 ± 57 | 3.1 | 0.66 |
| C17U | > 150,000b | > 800 | > 4.0 |
| C17G | > 150,000b | > 800 | > 4.0 |
| C15U | 1100 ± 71 | 5.8 | 1.0 |
| C15G | > 150,000b | > 800 | > 4.0 |
| A16U | 20,000 ± 460 | 110 | 2.8 |
| A91G | 100 ± 16 | 0.53 | −0.38 |
| A16U/A91G | 1400 ± 58 | 7.4 | 1.2 |
| C17U/A91G | 11,000 ± 500 | 58 | 2.4 |
All J1/2 mutations were made in the background of the C44U/G83A mutation.
No binding was detected at 150 μM RNA.
