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Binding affinities for P1 truncation mutants.
| RNA | Kd (nM) | ΔΔGbind (kcal/mol) |
|---|---|---|
| Wild-type | 0.011 | 0 |
|
| ||
| Vc2 Δ93 | 3400 ± 160 | 7.5 |
| Vc2 Δ94 | 280 ± 59 | 6.0 |
| Vc2 Δ95 | 63 ± 13 | 5.1 |
| Vc2 Δ96 | 5.4 ± 0.59 | 3.7 |
| Vc2 Δ97 | 4.6 ± 0.25 | 3.6 |
| Vc2 Δ98 | 5.9 ± 0.79 | 3.7 |
| Vc2 Δ99 | < 0.8a | < 2.5 |
| Vc2 Δ100 | < 0.5a | < 2.3 |
These values could not be measured using equilibrium methods and upper estimates are given.
