Table 5.
RNA | Kd (nM) | kon (M−1 min−1) | koff (min−1) |
---|---|---|---|
Wild-type | 0.011 | 1.0 ± 0.016 × 106 | 1.1 ± 0.082 × 10−5 |
| |||
C92Ua | 15 ± 1.1 | 8.0 ± 0.99 × 105 | 1.0 ± 0.16 × 10−2 |
G20A | 0.21 ± 0.067 | 9.1 ± 1.8 × 105 | 2.2 ± 0.077 × 10−4 |
| |||
C44U/G83A | 190 ± 8.6 | 8.7 ± 1.2 × 104 | 2.5 ± 0.25 × 10−2 |
| |||
Vc2 Δ95 | 63 ± 13 | 2 × 105 b | 1.3 ± 0.10 × 10−2 |
Vc2 Δ96 | 5.4 ± 0.59 | 2 × 106 b | 1.0 ± 0.10 × 10−2 |
Vc2 Δ98 | 5.9 ± 0.79 | 5.0 ± 1.0 × 105 | 3 × 10−3 b |
Vc2 Δ99 | < 0.8c | 1.3 ± 0.40 × 106 | - |
Vc2 Δ100 | < 0.5c | 1.2 ± 0.30 × 106 | - |
All values reported for C92U were reported previously (21).
Calculated from the equation Kd=koff/kon.
These values could not be measured using equilibrium methods and upper estimates are given.