Table 5.

Biochemical studies on anxiety disorders

Author and study	Sample	Methodology	Results and conclusions
Rao et al.,[61] A study of spontaneous functional hypoglycaemia in relation to anxiety	Series of 9 patients out of 42 patients of anxiety were included who have developed with predominant symptoms of anxiety related to food intervals	Complete psychiatric (history and MSE) and physical evaluation, routine urine examination, and GGT was done using the Somogyi-Nelson method. In this the last specimen of blood was taken at the end of 3 hours.	Two patients did not improve at all, 4 patients improved significantly and tranquillizers discontinued. Three patients showed some improvement and they were able to adjust, with weekly supportive therapy The spontaneous hypoglycemia of functional origin is not so uncommon. it is worth-while to do a G.T.T. in all cases of anxiety reaction arising in relation of food
Singh et al.[62] Response to pineal gland in clinical cases of psychological stress	Fifty patients of thyrotoxicosis (25) and anxiety neurosis (25) suffering from psychological stress	Biochemical estimation of melatonin was done in all these cases prior to treatment and 3 months after appropriate treatment.	A significant increase in the level of melatonin was observed in thyrotoxic and anxiety neurotic cases. In all these cases, after therapy the level of melatonin was found to be within the normal limits. These observations confirm the finding that there is a pineal response to psychic stress.
Mishra et al.[63]	36 patients of anxiety neurosis diagnosed as per Feighner’s diagnostic criteria (Feighner et al.),[64] attending the OPD were included with 24 control subjects.	The patients were assessed on Hamilton anxiety scale	Patients of anxiety neurosis showed hyper triglyceridemia and increased VLDL-cholesterol concentration with reduction in esterified cholesterol level. First two findings reflect that anxiety neurotics are at a greater risk for the development of atherosclerosis and its cardiovascular complications and that TGs, VLDL, cholesterol and esterified cholesterol are perhaps the biochemical variables mediating the cardiovascular psychosomatic response. The assumptions are tentative, and regretfully have never been studied again.