Fig. 3.
A, Maximal cAMP production (Rmax) of LHR+/Gαs+ wild-type and variant cells stimulated with CTX. Measurements were made after incubating cells for 2 h (37 C) with 2 μg/ml CTX, and the results are given as mean ± sem. One-way ANOVA followed by Dunnett's posttest was used for statistical analysis, and all samples were found to be significantly different from wild-type (wt) Gαs (P < 0.001). B, Basal cAMP values of the transfected s49 ck cells. The cells were incubated for 30 min, and one-way ANOVA followed by Dunnett's posttest was used for statistical analysis. *, P < 0.05; **, P < 0.001.