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. 2011 May 26;25(8):1301–1310. doi: 10.1210/me.2010-0455

Fig. 3.

Fig. 3.

Increased serum lactate level, decreased mitochondrial ATP production, and decreased electron transport chain complex I activity in TR4−/ − mice. A, Lactate content in the serum from seven pairs of TR4+/+ and TR4−/− mice was determined by measuring the oxidized DCPIP as described in Materials and Methods. TR4−/ − mice display elevated serum lactate levels as compared with TR4+/+ mice. B, Determination of ATP generation by a firefly luciferase-based assay. Mitochondria isolated from three pairs of TR4+/+ and TR4−/− skeletal muscle were subjected to ATP generation assay. TR4−/ − mice muscle exhibited markedly lower ATP production rate per gram mitochondrial protein compared with mitochondria from TR4+/+ mice. C, Determination of respiratory chain enzyme activity between TR4+/+ and TR4−/−. Mitochondria isolated from four pairs of TR4+/+ and TR4−/− skeletal muscle were applied to respiratory chain enzyme activity assays as described in Materials and Methods. Three independent experiments were carried out, and the mean ± sd was shown. **, P < 0.01. KO, Knockout; WT, wild type.