Table 2. Effects of data preprocessing on the significance (P-values) in resolving the differences of microbial communities between warming and unwarminga.
| Primers |
Not removing singletons |
Removing singletons |
||||
|---|---|---|---|---|---|---|
| Cut 0b | Cut 1c | Cut 2d | Cut 0b | Cut 1c | Cut 2d | |
| Forward | 0.55 | 0.05 | 0.03 | 0.44 | 0.07 | 0.02 |
| Reverse | 0.51 | 0.08 | 0.04 | 0.19 | 0.13 | 0.03 |
| Forward and reverse | 0.63 | 0.02 | 0.03 | 0.04 | 0.03 | <0.01 |
Abbreviations: ANOSIM, analysis of similarity; MRPP, multiresponse permutation procedure.
a
The values in the table are probability values (P), indicating significance with the Adonis method. Three statistical methods (MRPP, ANOSIM and Adonis) were used. However, Adonis is more sensitive; thus, only results using this method are presented.
b
No OTUs were removed.
c
OTUs present only in 1 of the 14 tag sequence data sets of a treatment were removed.
d
OTUs present in 1 or 2 of the 14 tag sequence data sets of a treatment were removed.