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. 2011 Jan 20;5(7):1162–1177. doi: 10.1038/ismej.2010.208

Table 1. Primers used in this study.

Primer Sequence Primer Expected product size
intI-864Ra 5′-YAGCAGATGNGTGGCRAAVSWRTGSCG-3′ GCP2 Varied
GCP1b 5′-GCSGCTKANCTCVRRCGTTRRRY-3′ GCP2 Varied
GCP2b 5′-TCSGCTKGARCGAMTTGTTRRRY-3′    
27Fc 5′-AGAGTTTGATCCTGGCTCAG-3′ 1492R ∼1500 bp
1492Rc 5′-GGTTACCTTGTTACGACTT-3′    
a

Primer targets integrase gene intl.

b

Primer targets gene cassette base element attC.

c

Primer targets 16S rRNA gene.