Fig. 4.

Infection of HCASMCs with AdSP-D increases the production of SP-D protein. Cultured HCASMCs were infected with 0–100 multiplicity of infection (MOI) of AdSP-D as described in MATERIALS AND METHODS. A: SP-D protein expression in media and cells following adenoviral gene transfer. After 48 h, culture media (lanes 1–6) and cell extracts (lanes 7 and 8) were immunoblotted for SP-D protein, which was quantitated by densitometry and expressed as relative optical density (OD; B). Two immunoreactive bands were detected in the media; the upper band was produced by cross-reactivity with a protein in the serum-containing culture medium. The lower band corresponds to authentic human SP-D protein. The data in B are representative of 2 experiments; levels of SP-D are expressed relative to control. C: uniform cellular distribution of SP-D protein in the cytoplasm of HCASMCs, as detected by confocal microscopy.