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SP-D mRNA levels are increased in HCASMCs by LPS and TNF-α. HCASMCs were treated with either LPS (2 ng/ml, solid line) or TNF-α (2 ng/ml, dashed line) for 2–24 h. SP-D mRNA levels were assessed by real-time PCR. Data were normalized to the level of SP-D mRNA in control (untreated) cells harvested at time 0. Data represent the average increase in the relative amount of SP-D mRNA in 2 experiments with duplicate samples collected at each time point.
