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Cartoon depicting a method to calibrate reporter concentration in cells. A drop of purified fluorophore of known concentration (grey color) is placed in a wedge generated using coverslips. A series of images is acquired across the length of the wedge to correlate the intensity of signal with the height at each point in the wedge. From this and knowing the estimated thickness of the cell type used, one can estimate the intensity expected for reporter concentration within a cell.
