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. Author manuscript; available in PMC: 2011 Jul 29.
Published in final edited form as: Curr Protoc Chem Biol. 2009 Dec 1;1(1):17–18. doi: 10.1002/9780470559277.ch090106

Figure 3.

Figure 3

Quantitation of the basal, stimulated, and phosphatase-suppressed PKC activities at specific regions, showing the range of the C Kinase Activity Reporter (CKAR) at each cellular location. The reporter has a consistent maximal range (20% FRET ratio change) at plasma membrane (PM), Golgi, cytosol and nucleus, but a reduced range at the outer membrane of mitochondria. (Figure from Gallegos et al., 2006).