Table 1.
Oligonucleotides used for amplification of the haemagglutinin and neuraminidase genes of H5N1
| Name | Sense | Primer sequence (5'-3') | Product size | Reference |
|---|---|---|---|---|
| HA a | For | agcraaagcaggggt | 699 | |
| Rev | ctctgrttyagtgttgatgt | FLI -Germany* | ||
| HA b | For | gagcagaataaaycattttgaga | 801 | |
| Rev | tgagtggattctttgtctgcagc | |||
| HA c | For | acatcaacactraaycagag | 716 | |
| Rev | aagtctagagttctctcattyt | |||
| HA d | For | gctgcagacaaagaatccactca | 615 | |
| Rev | gaccagtagaaacaagggtgtttt | |||
| NA | For | atgaatccaaatcagaag | 1345 | [13] |
| Rev | tgtcaatggtgaatggcaac |
*Primer sequences for HA (a-d) were ordered according to information provided by Dr. Sasan R Fereidouni, the Laboratory of Molecular and Biological Characterization of AIV, Friedrich Loeffler Institute, Germany.