. 2011 Jun 8;8:283. doi: 10.1186/1743-422X-8-283

Table 2.

Comparative representation of the distribution of haemagglutinin epitopes that showed variation among avian and human H5N1 Egyptian isolates

Strain*	A EPITOPE

	110	115	117	119	121	122	124	126	127	129	130	136	152
F7	H	Q	I	K	S	W	D	E	A	Δ	G	P	K
F8	H	Q	I	K	S	W	D	E	A	Δ	G	P	K
F9	H	Q	I	K	S	W	D	E	A	S	G	P	K
F10	R	Q	I	K	S	W	D	E	A	L	G	P	K
2006	H	Q	I	K	S	W	D³⁶/N¹	E	A	S	G	P	K
2007	H⁷¹/R⁷	Q⁷⁷/K¹	I⁷⁶/F²	K⁷²/N⁶	S⁷⁷/C¹	W	D⁶³/N¹⁴/H¹	E	A⁷⁵/T³	S⁶⁶/L⁶/Δ⁶	G	P	K
2008	H³²/R⁵⁰	Q⁸¹/H¹	I⁷⁵/F⁷	K⁸⁰/R²	S⁸⁰/P¹/Y¹	W	D⁶⁷/N¹⁵	E	A	S⁶⁶/L¹⁶	G⁸¹/R¹	P⁷⁷/Q³/S²	K
2009	H⁵⁰/R²⁴	Q⁶⁹/K⁵	I⁶⁷/F⁴/T³	R⁷³/S¹	S⁷³/Y¹	W	D⁷²/N²	E	A	Δ³⁶/L²²/S¹⁶	G	P⁷²/Q²	K⁷⁰/E⁴
2010	H²⁷/R¹⁷	Q³⁴/K¹⁰	I⁴³/T¹	K	S	W⁴³/C¹	D⁴²/N²	E⁴³/G¹	A	Δ²⁶/L¹⁸	G	P	K³⁹/E⁴/G¹
Human	H⁹³/R¹	Q	I	K	S	W	D⁸⁸/N⁶	E⁹³/G¹	A⁹³/T¹	Δ⁵⁰/L¹/S⁴³	G	P	K⁹²/Q²

Strain	B EPITOPE									C EPITOPE

	139	140		141	144	147	174		178	181	30		257	263	278

F7	G	R		S	F	V	V		I	P	Q		V	T	C
F8	G	R		S	F	V	V		I	P	Q		V	T	C
F9	G	R		S	F	V	V		I	P	Q		V	T	C
F10	G	G		P	Y	V	V		I	P	Q		V	T	C
2006	G	G		S³⁵/A²	F	V	V		I	P	Q		V	T	C
2007	G	R⁵⁹/K⁸/G⁷I³/S¹		S⁶⁴/P¹⁴	F⁷¹/Y⁷	V⁷⁶/M²	V⁷⁷/I¹		I	P	Q		V	T	C
2008	G	G⁴⁷/R³⁴/E¹		P⁴⁹/S³⁰/T²/L¹	F³²/Y⁵⁰	V⁸⁰/M²	V⁸⁰/I²		I⁸¹/V¹	P⁸¹/T¹	Q⁸⁰/K²		V	T⁸¹/A¹	C
2009	G	R⁵¹/G²⁰/E¹I¹/S¹		S⁴⁶/P²³/T²/L³	F⁵¹/Y²³	V	V⁶⁹/I⁵		I	P⁷³/S¹	Q		V⁷³/I¹	T	C⁷³/F¹
2010	G⁴³/R¹	S²⁸/P¹⁰/L⁵/T¹		S²⁸/P¹⁰/L⁵/T¹	F²⁸/Y¹⁶	V	V⁴²/I²		I	P	Q		V	T	C
Human	G	R⁹¹/G²/K¹		S⁹¹/P³	F⁹³/Y¹	V	V⁹²/I²		I	P	Q		V	T	C

	EPITOPE D

	86	101	105	151	154	155	156	158	159	161	163		185	192

F7	A	L	L	T	D	N	A	P	T	K	S		T	Q
F8	A	L	L	T	D	N	A	P	T	K	S		T	Q
F9	A	L	L	I	N	N	A	P	T	K	S		A	H
F10	A	L	L	I	N	N	T	P	T	K	S		E	K
2006	A	L	L	I	D³⁴/N³	N	A	P	T	K	S		A	Q
2007	A⁷⁷/V¹	L⁷⁷/M¹	L⁷⁷/F¹	T⁶/I⁷²	D⁶⁵/N¹⁰/G²/B¹	N	A⁷⁷/V¹	P	T	K	S		A	Q
2008	A⁷⁹/S²/V¹	L	L⁸¹/S¹	T⁴/I⁷⁷/V¹	D⁶²/N¹⁶/G³/E¹	N⁸¹/T¹	A⁶⁶/T¹⁶	P	T	K⁷⁶/E⁵/T¹	S⁷⁷/N²/T²/R¹		A⁷²/E⁷/T³	Q⁶⁸/K¹⁴
2009	L⁶⁹/V⁵	L	L	T³⁶/I³⁰/L⁴/V⁴	D⁴³/N³⁰/G¹	N⁷¹/S²/D¹	A⁵¹/T²³	P	T⁶⁹/L³/I²	K⁷³/R¹	S⁷³/N¹		A⁵²/E²⁰/T²	Q⁴⁷/K²¹/H⁶
2010	L⁴²/S¹/T¹	L⁴³/M¹	L	T²⁸/I¹⁶	N³²/D¹²	N⁴³/D¹	A²⁷/T¹⁷	P⁴³/L¹	T³⁹/L⁵	K	S³⁹/N⁵		A²⁸/E¹²/T³/V¹	Q²⁷/K¹⁷
Human	A⁹⁰/S²/T²	L	L	T⁵⁰/I⁴⁴	D⁶¹/N²⁹/G³/E¹	N⁹³/S¹	A⁹³/T¹	P	T	K	S		A⁸⁸/E³/T³	Q⁸⁹/H⁴/K¹

Strain	EPITOPE D									EPITOPE E

	197	198	199	210	212	226	43	47	66	71	72	75	83	244	249

F7	Y	I	S	V	K	M	N	V	M	L	N	E	I	N	A
F8	Y	I	S	V	K	M	N	V	M	L	N	E	I	N	A
F9	Y	I	S	V	K	M	D	V	M	L	N	E	I	N	A
F10	Y	I	S	V	K	V	D	V	M	P	N	E	I	N	A
2006	Y	I	S	V	K	M³⁵/I²	D	V	M	L	N	E	I	N	A
2007	Y⁷⁷/Q¹	I	S	V	K	M⁴⁶/V⁷/I²⁵	D⁷²/N⁶	V⁷⁷/M¹	M⁷⁷/L¹	L⁷⁷/H¹	N⁷²/D⁶	E	I⁷⁷/V¹	N	A
2008	Y	I	S⁸⁰/P¹	V⁷⁶/I⁶	K⁸⁰/T¹	M²³/V⁴⁹/I¹⁰	D⁷⁸/N⁴	V	M	L⁷³/P⁵/F⁴	N	E⁸¹/K¹	I	N	A
2009	Y	I	S	V	K⁷⁰/R⁴	M⁴³/V²³/I⁸	D³⁸/N³⁶	V	M	L⁵⁵/P¹⁹	N	E	I⁶⁸/T⁴/S²	N⁷³/D¹	A
2010	Y	I⁴³/V¹	S	V⁴³/A¹	K	M²⁷/V¹⁷	D¹⁷/N²⁶/S¹	V	M	L³²/P¹²	N	E	I³⁹/T⁵	N	A⁴³/V¹
Human	Y	I	S	V⁹³/I¹	K⁹³/Q¹	M⁹⁰/V¹/I³	D⁴⁴/N⁵⁰	V⁹³/M¹	M	L	N	E	I	N	A

*: Egyptian; avian strain from tested (F7-F10) and published sequences in the flu database from 2006-2010 as well as Egyptian human published sequences (2006-2010).

Epitopes allocation were determined previously by Duvvuri et al [16].

Δ: Deletion,

Superscripts denote the number of isolates that possess the specified amino acid residue.

Avian isolates: 2006: n=37, 2007: n=78, 2008: n=82, 2009:n=74, 2010:n=44 Human isolates: n=94.