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. 2011 Jul 29;6(7):e21696. doi: 10.1371/journal.pone.0021696

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Yanhua Liang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Sharpin-flag, pcDNA-Traf2, pcDNA-Traf6 were respectively transfected into wildtype or Sharpin^cpdm/cpdm mutant mouse embryonic fibroblasts using different combinations. After selection and confirmation, a luciferase labeled NFκB plasmid was transfected into the cells to quantify chang.