Figure 1. Sequence and analysis of ChTFF2.

A: Strategy of amplying and sequencing full length ChTFF2 cDNA. B: RLM-RACE products were electrophoresed in a 2% agarose gel. Lane 1: 3′ RACE product; lane 2: water control; lane 3: 5′ RACE product; lane 4: water control. D: Intron-exon localization of ChTFF2 at chromosome. C: Representative electrophoresis result from EcoRI digestion of purified plasmids inserted with 5′ RACE product indicates alternative start site with shorter product in lane 2 as compared with lane 1 and 3; alternative transcription starting site was confirmed by sequencing determination. The ORFs are gray. E: Potential regulatory sequences in the promoter of the ChTFF2 as compared with human; nucleotides are numbered with +1 being the A of the ATG initiation codon; target sequences for the underlined transcription factors (motif) are in bold. F: Full-length ChTFF2 cDNA sequence and deduced amino acids.