Figure 3. Spatio-temporal expression of TFF2 transcripts in chicken.

Representative amplification plots of quantitative PCR (duplicates) for measuring ChTFF2 (A) and 18S (B) in the small intestine; water was used as a control. Quantification of ChTFF2 transcripts in embryonic (E) and post-hatch (H) ages in duodenum (C), jejunum (D) and ileum (E) using RT-qPCR; values were expressed in mean ± SE, n = 5 to 8. The expression of ChTFF2 (F) and 18S (G) in different tissues; forty ng of cDNA were amplified for 33 cycles using a forward primer from RT-PCR1and a reverse primer from RT-PCR2; reverse transcriptase was used (+) or omitted (−) for each tissue and all the RT-PCR products were examined by electrophoresis through a 3% agarose gel in TBE.