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. 2011 Jul 29;6(7):e22785. doi: 10.1371/journal.pone.0022785

Figure 1. Yeast two-hybrid screen identifies UBC9 as a PR-Set7-interacting protein.

Figure 1

(A) Schematic representation of the different Gal4-DBD-PR-Set7 bait proteins used for the yeast two-hybrid screen and the recovered AD-UBC9. (B) The indicated plasmids were co-transformed into AH109 yeast strain and grown on medium lacking: tryptophan and leucine (DDO), and histidine (TDO) and adenine with addition of X-alpha-galactosidase (QDO). Growth on TDO and QDO indicates interaction between PR-Set7 and UBC9. (C) Western analysis of HA-immunoprecipitations from yeast co-transformants expressing the indicated plasmids using PR-Set7 and UBC9 antibodies on the input (In), unbound (U) and bound (B) fractions.