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. 2011 Jul 29;6(7):e22785. doi: 10.1371/journal.pone.0022785

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Spektor et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) HEK 293 cell lysates expressing FLAG-PR-Set7 or FLAG-null were immunoprecipitated with an UBC9 antibody. Western analysis of the input (In) and bound (B) fractions were analyzed using FLAG or UBC9 antibodies. (B) HEK 293 cells co-transfected with the indicated plasmids were crosslinked using 10 µM BMH prior to FLAG-immunoprecipitations. Western analysis was performed using Myc or FLAG antibodies on the input (In), unbound (U) and bound (B) fractions.