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. 2011 Jul 29;6(7):e22785. doi: 10.1371/journal.pone.0022785

Figure 7. Reduction of UBC9 results in derepression of PR-Set7-regulated genes.

Figure 7

HEK 293 cells were transfected with a null shRNA plasmid or shRNA plasmids designed to reduce PR-Set7 or UBC9 levels. Quantitative RT-PCR and Western analysis were performed to validate the reduced transcript and protein, respectively, of PR-Set7 (A) or UBC9 (C). Quantitative RT-PCR was performed to analyze the expression levels of three newly identified PR-Set7-regulated genes or the CBR1 negative control gene in null shRNA (black bar), PR-Set7 shRNA (B) or UBC9 shRNA (D) cells (grey bar). Results were normalized to 18S expression and plotted as the fold increase relative to null shRNA. Three technical replicates were used to generate standard deviations and statistical significance was calculated using the student-T test (asterisks are p≤0.05). Similar results were obtained in independent biological replicates.