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. 2011 Jul 29;6(7):e22762. doi: 10.1371/journal.pone.0022762

Figure 1. The ccp mutant has no detectable protease activity.

Figure 1

Culture supernatants (n = 4) isolated at 3.5 h from the wild-type strain JIR325 (WT), a ccp mutant carrying the vector plasmid pJIR750 M(v) (JIR12503), and the ccp mutant carrying the ccp+ complementation plasmid pJIR3680, M(ccp+) (JIR12501) were assayed for protease activity as previously described [26] and results expressed as the rate of azo-dye release/minute/mg protein. Error bars indicate S.E.M (*** p<0.0001) using a two-tailed unpaired student's t-test comparing the WT and M(v).