Figure 4. Expression of E-cadherin, CK, α-SMA, vimentin in HK-2 cells by immunochemistry.

(A–C)The HK-2 cells under 30 mM glucose(B) or 30 mM glucose+cont siRNA(C) showed a loss of CK and E-cadherin and an increase of α-SMA and vimentin expression compared to that of the cells under 5.5 mM glucose condition(A).(D–F)These changes were prevented by exposed to 30 mM glucose+p38siRNA for 24 h(D) or 48 h(E) or+AP-1 inhibitor(F).