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. 2011 Jul 29;6(7):e22806. doi: 10.1371/journal.pone.0022806

Figure 6. Effect of knockdown of p38MAPK by p38 MAPK siRNA on p38 MAPK expression by Western blot and RT-PCR analysis.

Figure 6

(A)P38 MAPK expression were determined by RT-PCR of the HK-2 cells cultured for 72 h in 5.5 mM glucose (lane 1), 5.5 mM glucose+Cont siRNA (lane 2), 5.5 mM glucose+p38 siRNA (lane 3). (B)P38 MAPK expression were determined by Western blot of the HK-2 cells cultured for 72 h in 5.5 mM glucose (lane 1), 5.5 mM glucose+Cont siRNA (lane 2), 5.5 mM glucose+p38 siRNA (lane 3).Values represent the mean ± SD, aP<0.05 vs. 5.5 mM glucose.