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. 2011 Jul 29;6(7):e22806. doi: 10.1371/journal.pone.0022806

Figure 10. Activity of AP-1 in HK-2 cells by EMSA.

Figure 10

(A)AP-1 activity was analyzed by EMSA of the HK-2 cells cultured in 5.5 mM glucose (lane 1), 30 mM glucose(lane 2), 30 mM glucose+Cont siRNA(lane 3), 30 mM glucose+p38 siRNA for 24 h (lane 4), 30 mM glucose+p38 siRNA for 48 h(lane 5).(B) AP-1 activity was analyzed by EMSA of the HK-2 cells cultured in 5.5 mM glucose(lane 1), 30 mM glucose (lane 2) and 30 mM glucose+AP-1 inhibitor(lane 3). Values represent the mean ± SD, aP<0.05 vs. 5.5 mM glucose, bP<0.05 vs. 30 mM glucose.