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. 2011 Jul 29;6(7):e22953. doi: 10.1371/journal.pone.0022953

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Siebert et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Expression analysis of isogroup03256 in developing nectophores (A) and gastrozooids (B). The transcript is localized in the basigaster, a region associated with nematogenesis, at the base of gastrozooids and detectable in very early stages of gastrozooid development (arrow). (C) Sense control with an unstained basigaster region. In situ hybridizations for nectophore specific transcripts have been performed successfully (data not shown). S: stem of the colony, b: basigaster region of the gastrozooid, pn: pneumatophore.