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. 2011 Jul 29;6(7):e22986. doi: 10.1371/journal.pone.0022986

Figure 3. The DNA damage checkpoint sensors 9-1-1, ATRIP, and XPA preferentially bind branched DNA.

Figure 3

The association of each of the indicated purified proteins with beads alone (No DNA) or to single-stranded (ss), double-stranded (ds), and branched, replication fork-like (fork) DNA bound to magnetic beads was analyzed by SDS-PAGE and immunoblotting. Each of the following proteins were analyzed for binding to each DNA structure: (A) 9-1-1, (B) ATRIP, and (C) XPA. Reactions were carried out as described in the Methods section.