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. 2011 Jul 29;6(7):e22986. doi: 10.1371/journal.pone.0022986

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Yilmaz et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The association of each of the indicated purified proteins with beads alone (No DNA) or to single-stranded (ss), double-stranded (ds), and branched, replication fork-like (fork) DNA bound to magnetic beads was analyzed by SDS-PAGE and immunoblotting. Each of the following proteins was analyzed for binding to each DNA structure: (A) TopBP1 and (B) Cdc45. Reactions were carried out as described in the Methods section.