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. 2011 Jul 29;6(7):e22986. doi: 10.1371/journal.pone.0022986

Figure 7. RPA, ATRIP, and XPA show a slight preference for AAF-damaged DNA.

Figure 7

The association of each of the indicated purified proteins with beads alone (No DNA) or to undamaged (−AAF) or N-acetoxy-2-acetylaminofluorene (AAF)-treated (+AAF) plasmid DNA bound to magnetic beads was analyzed by SDS-PAGE and immunoblotting. Each of the following proteins was analyzed for binding to each DNA structure: (A) RPA, (B) ATRIP, and (C) XPA. Reactions were carried out as described in the Methods section.