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The association of each of the indicated purified proteins with beads alone (No DNA) or to undamaged (−AAF) or N-acetoxy-2-acetylaminofluorene (AAF)-treated (+AAF) plasmid DNA bound to magnetic beads was analyzed by SDS-PAGE and immunoblotting. Each of the following proteins was analyzed for binding to each DNA structure: (A) Claspin and (B) Tipin. Reactions were carried out as described in the Methods section.
