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The association of each of TopBP1 with beads alone (No DNA) or to undamaged (−AAF) or AAF-damaged (+AAF) plasmid DNA bound to magnetic beads was analyzed by SDS-PAGE and immunoblotting. Binding reactions contained either (A) 100 mM NaCl, (B) 200 mM NaCl, or (C) 300 mM NaCl. Reactions were carried out as described in the Methods section.
