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. 2011 Jul 29;6(7):e23107. doi: 10.1371/journal.pone.0023107

Figure 4. Analysis of endogenous MCM4 expression in Leishmania promastigotes.

Figure 4

A. Western blot analysis of extracts from proliferating promastigotes. Extracts (10% SDS-PAGE) probed with anti-MCM4 antibody (1∶1000 dilution), anti-PCNA antibody (1∶5000 dilution; loading control for nuclear extracts), anti-tubulin antibody (1∶2000 dilution; loading control for cytosolic extracts). CE- cytosolic extract; NE- nuclear extract. B. MCM4 expression at different stages of the cell cycle. Upper panel – Flow cytometry profiles of cells harvested at different timepoints. Lower panel - Western blot analysis of nuclear extracts (4×107 cell equivalents) using anti-MCM4 antibody (1∶1000 dilution) and anti-PCNA antibody (1∶5000 dilution; loading control). 1- log; 2- HU block; 3- S phase; 4- late S phase; 5- G2/M phase. Bar chart represents expression of MCM4 relative to PCNA. Arrowheads indicate MCM4.