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. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: DNA Repair (Amst). 2011 May 31;10(8):826–834. doi: 10.1016/j.dnarep.2011.04.032

Figure 4. Bypass of UV photoproducts and AP sites by L979F Pol ζ when dNTP concentrations are at normal levels.

Figure 4

Images of denaturing polyacrylamide gels showing products of synthesis by L979F Pol ζ with (A) undamaged, CPD and (6-4) PP 49-mer substrates, (B) CPD and (6-4) PP 45-mer substrates, and (C) undamaged, TAF and TAG substrates. Each primer extension reaction contained an 8-fold excess of substrate over polymerase and normal dNTPs concentrations. The average bypass probability and the relative bypass efficiency (bypass of damaged substrate relative to bypass of undamaged substrate) for each reaction are shown under the gel. Markings are as in Figure 1.