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. Author manuscript; available in PMC: 2012 Aug 19.
Published in final edited form as: J Mol Biol. 2011 Jun 7;411(3):661–679. doi: 10.1016/j.jmb.2011.05.047

Fig. 9.

Fig. 9

Northern hybridization and correlated immunoblot comparing the ability of wild-type and mutant Mss116p to promote splicing of aI5γ in vivo. (a) Northern hybridization. The blot shows whole-cell RNAs (1.0 μg) from the indicated strains separated in a 1.5% agarose gel and hybridized with a 32P-labeled oligonucleotide complementary to COX1 exon 6. Lanes: (1) WT 161-aI5γ (WT); (2) mss116Δ-aI5γ transformed with CEN plasmid pRS416 (empty vector); (3–6) mss116Δ-aI5γ transformed with CEN plasmids expressing Mss116p mutants K158A, K158R, and SAT/AAA. (b) Immunoblot. The blot shows TCA-precipitated proteins (~60 μg) from the same strains as in panel (a) separated in a 4–20% polyacrylamide gradient gel and probed with an anti-Mss116p antibody. (c) Immunoblot stained with AuroDye Forte to confirm equal loading. The numbers to the left of the gel in (b) and (c) indicate the positions of size markers (Precision Plus Protein Dual Color Standards; Bio Rad).